The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.
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一场罕见病攻坚2021年,德国Georg Schett教授团队首次使用抗CD19 CAR-T成功治疗了一位难治性系统性红斑狼疮患者,这一里程碑事件如同投入湖面的石子,激起了整个行业的巨大涟漪。。关于这个话题,爱思助手下载最新版本提供了深入分析
Фото: Planet Labs PBC / Handout via Reuters,详情可参考clash下载 - clash官方网站